Research on Biomedical Engineering
http://www.rbejournal.periodikos.com.br/article/doi/10.4322/rbeb.2013.047
Research on Biomedical Engineering
Original Article

FT-Raman spectroscopic study of skin wound healing in diabetic rats treated with Cenostigma macrophyllum Tul

Coelho, Nayana Pinheiro M. de F.; Raniero, Leandro; Costa, Charlytton Luis S. da; Maia Filho, Antonio Luis M.; Martins, Marcelino; Martin, Airton Abrahão; Arisawa, Emilia Ângela L.

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Abstract

Introduction: Patients with diabetes mellitus exhibit a delay in the lesion repair process. The active components of Cenostigma macrophyllum may represent a viable alternative to facilitate the recovery of these lesions. The aim of this study was to evaluate the effects of emulsion oil-water Cenostigma macrophyllum in the repair process of lesions in rats with induced diabetes. Methods: 63 male rats (Wistar, 200-250 g body weight, 30-40 days old) were distributed into the following groups: control (C), diabetic (D) and diabetic treated with Cenostigma macrophyllum (P), subdivided based on the experimental times, days 7, 14 and 28, with 21 animals per main group. Diabetes mellitus (DM) was induced by administration of streptozotocin (50 mg/kg via penile vein and 12-h fasting) and confirmed at day 21 (glycemic index > 240 mg/dL). In the animals of group P, 0.5 ml of the oil-water emulsion obtained from the plant seed was used. The samples were removed and hemisectioned, and one portion was used for the quantitative histological analysis of collagen using Masson’s trichrome staining, while another portion was analyzed by FT-Raman spectroscopy. Results: A higher percentage area of the volume of collagen fibers was observed for the experimental time Day 14 in group P compared with group D (p < 0.001). Regarding the ratio of areas of the amides I (1700‑1600 cm–1) and III (1245-1345 cm–1), the groups D and P show the opposite behavior. Conclusion: Cenostigma macrophyllum accelerated the repair process in skin of diabetic ratsfor14 days.

Keywords

Cenostigma macrophyllum, Raman spectroscopy, Tissue repair
5889fbe05d01231a018b482f rbeb Articles
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Res. Biomed. Eng.

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