Research on Biomedical Engineering
http://www.rbejournal.periodikos.com.br/article/doi/10.1590/2446-4740.180039
Research on Biomedical Engineering
Technical Communication

Production of fibrous polymer scaffolds for tissue engineering using an automated solution blow spinning system  

Alessandra Forgatti Hell, Márcia Mayumi Omi Simbara, Paulo Rodrigues, Danilo Akio Kakazu, Sônia Maria Malmonge

Downloads: 0
Views: 79

Abstract

Introduction: Solution blow spinning (SBS) and airbrushing are two techniques that can be used as alternatives to electrospinning in the production of fibrous scaffolds for tissue engineering (TE). SBS seems particularly interesting due to its versatility, however, it has not been much explored and no automated SBS systems were found in the literature. Therefore, the present work aimed to develop such equipment and compare the results to those found for airbrushing, considering the same set of parameters. Methods: A new SBS set up, composed of a specially designed nozzle with automated movement, a syringe pump and a compressor, was used to produce fibrous poly (ε-caprolactone) (PCL) mats. The airbrushed fibers were produced under the same conditions, and samples of both types of mats were imaged using scanning electron microscopy (SEM) to compare them in terms of
microstructure and fiber diameter. Results: The SBS system was robust and performed well, in terms of movement and fiber deposition. In comparison to airbrushing’s, SBS mats presented different microstructural characteristics (considering the parameters used). Conclusion: The biggest advantage over airbrushing may be its versatility and simple automation, which may improve sample reproducibility, especially considering scaled up processes. To further improve this apparatus, a better understanding of how process variables interfere in the microstructure is needed, as well as more sophisticated interface and operation.

Keywords

Fibrous scaffolds, Solution blow spinning, Airbrushing.

References

Barnes CP, Sell SA, Boland ED, Simpson DG, Bowlin GL. Nanofiber technology: designing the next generation of tissue engineering scaffolds. Adv Drug Deliv Rev. 2007; 59(14):1413-33. http://dx.doi.org/10.1016/j.addr.2007.04.022. PMid:17916396.

Daristotle JL, Behrens AM, Sandler AD, Kofinas P. A review of the fundamental principles and applications of solution blow spinning. ACS Appl Mater Interfaces. 2016; 8(51):34951-63. http://dx.doi.org/10.1021/acsami.6b12994. PMid:27966857.

Medeiros ES, Glenn GM, Klamczynski AP, Orts WJ, Mattoso LHC. Solution blow spinning: a new method to produce micro- and nanofibers from polymer solutions. J Appl Polym Sci. 2009; 113(4):2322-30. http://dx.doi.org/10.1002/app.30275.

Oliveira JE, Moraes EA, Costa RGF, Afonso AS, Mattoso LHC, Orts WJ, Medeiros ES. Nano and submicrometric fibers of poly(d,l-lactide) obtained by solution blow spinning: process and solution variables. J Appl Polym Sci. 2011; 122(5):3396-405. http://dx.doi.org/10.1002/app.34410.

Püspöki Z, Storath M, Sage D, Unser M. Transforms and operators for directional bioimage analysys: A survey. In: De Vos WH, Munck S, Timmermans J-P, editors. Advances in anatomy, embryology and cell biology: focus on bio-image informatics. Switzerland: Springer International Publishing; 2016. p. 69-93. http://dx.doi.org/10.1007/978-3-319-28549-8_3.

Rezakhaniha R, Agianniotis A, Schrauwen JTC, Griffa A, Sage D, Bouten CVC, van de Vosse FN, Unser M, Stergiopulos N. Experimental investigation of collagen waviness and orientation in the arterial adventitia using confocal laser scanning microscopy. Biomech Model Mechanobiol. 2012; 11(3-4):461-73. http://dx.doi.org/10.1007/s10237-011-0325-z. PMid:21744269.

Srinivasan S, Chhatre SS, Mabry JM, Cohen RE, McKinley GH. Solution spraying of poly(methyl methacrylate) blends to fabricate microtextured, superoleophobic surfaces. Polymer (Guildf). 2011; 52(14):3209-18. http://dx.doi.org/10.1016/j.polymer.2011.05.008.

Tutak W, Sarkar S, Lin-Gibson S, Farooque TM, Jyotsnendu G, Wang D, Kohn J, Bolikal D, Simon CG Jr. The support of bone marrow stromal cell differentiation by airbrushed nanofiber scaffolds. Biomaterials. 2013; 34(10):2389-98. http://dx.doi.org/10.1016/j.biomaterials.2012.12.020. PMid:23312903.

5ba6ee690e88250b7436c1ed rbejournal Articles
Links & Downloads

Res. Biomed. Eng.

Share this page
Page Sections