Research on Biomedical Engineering
http://www.rbejournal.periodikos.com.br/article/doi/10.1590/2446-4740.01215
Research on Biomedical Engineering
Technical Communication

Application of post-discharge region of atmospheric pressure argon and air plasma jet in the contamination control of Candida albicans biofilms

Doria, Anelise Cristina Osório Cesar; Sorge, Camila Di Paula Costa; Santos, Thaisa Baesso; Brandão, Jhonatan; Gonçalves, Polyana Alves Radi; Maciel, Homero Santiago; Khouri, Sônia; Pessoa, Rodrigo Sávio

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Abstract

Introduction: Candida species are responsible for about 80% of hospital fungal infections. Non-thermal plasmas operated at atmospheric pressure are increasingly used as an alternative to existing antimicrobial strategy. This work investigates the action of post-discharge region of a non-thermal atmospheric plasma jet, generated by a gliding arc reactor, on biofilms of standard strain of Candida albicans grown on polyurethane substrate. Methods: Samples were divided into three groups: (i) non-treated; (ii) treated with argon plasma, and (iii) treated with argon plus air plasma. Subsequently to plasma treatment, counting of colony-forming units (CFU/ml) and cell viability tests were performed. In addition, the surface morphology of the samples was evaluated by scanning electron microscopy (SEM) and optical profilometry (OP). Results: Reduction in CFU/ml of 85% and 88.1% were observed in groups ii and iii, respectively. Cell viability after treatment also showed reduction of 33% in group ii and 8% in group iii, in comparison with group i (100%). The SEM images allow observation of the effect of plasma chemistry on biofilm structure, and OP images showed a reduction of its surface roughness, which suggests a possible loss of biofilm mass. Conclusion: The treatment in post-discharge region and the chemistries of plasma jet tested in this work were effective in controlling Candida albicans biofilm contamination. Finally, it was evidenced that argon plus air plasma was the most efficient to reduce cell viability.

Keywords

Gliding arc plasma, Biofilm, Candida albicans, Cell viability.

References

Alkawareek MY, Algwari QT, Laverty G, Gorman SP, Graham WG, O’Connell D, Gilmore BF. Eradication of Pseudomonas aeruginosa biofilms by atmospheric pressure non-thermal plasma. PloS One. 2012; 7(8):e44289. PMid:22952948.

Chen C, Liu DX, Liu ZC, Yang AJ, Chen HL, Shama G, Kong MG. A model of plasma-biofilm and plasma-tissue interactions at ambient pressure. Plasma Chemistry and Plasma Processing. 2014; 34(3):403-41. http://dx.doi.org/10.1007/s11090-014-9545-1.

Gaunt LF, Beggs CB, Georghiou GE. Bactericidal action of the reactive species produced by gas-discharge nonthermal plasma at atmospheric pressure: a review. IEEE Transactions on Plasma Science. 2006; 34(4):1257-69. http://dx.doi.org/10.1109/TPS.2006.878381.

Kong MG, Kroesen G, Morfill G, Nosenko T, Shimizu T, van Djik J, Zimmermann JL. Plasma medicine: an introductory review. New Journal of Physics. 2009; 11(11):1-35. http://dx.doi.org/10.1088/1367-2630/11/11/115012.

Laroussi M. Nonthermal decontamination of biological media by atmospheric-pressure plasmas: review, analysis, and prospects. IEEE Transactions on Plasma Science. 2002; 30(4):1409-15. http://dx.doi.org/10.1109/TPS.2002.804220.

Laroussi M, Leipold F. Evaluation of the roles of reactive species, heat, and uv radiation in the inactivation of bacterial cells by air plasmas at atmospheric pressure. International Journal of Mass Spectrometry. 2004; 233(1-3):81-6. http://dx.doi.org/10.1016/j.ijms.2003.11.016.

Ma Y, Zhang G-J, Shi X-M, Xu G-M, Yang Y. Chemical mechanisms of bacterial inactivation using dielectric barrier discharge plasma in atmospheric air. IEEE Transactions on Plasma Science. 2008; 36(4):1615-20. http://dx.doi.org/10.1109/TPS.2008.917165.

Mai-Prochnow A, Murphy AB, McLean KM, Kong MG, Ostrikov K. Atmospheric pressure plasmas: infection control and bacterial responses. International Journal of Antimicrobial Agents. 2014; 43(6):508-17. http://dx.doi.org/10.1016/j.ijantimicag.2014.01.025. PMid:24637224.

Ruiz LS, Khouri S, Hahn RC, Silva EG, Oliveira VK, Gandra RF, Paula CR. Candidemia by species of the Candida parapsilosis complex in children’s hospital: prevalence, biofilm production and antifungal susceptibility. Mycopathologia. 2013; 175(3-4):231-9. http://dx.doi.org/10.1007/s11046-013-9616-5. PMid:23404576.

Saad-Hossne R, Saad-Hossne W, Prado RG. Efeito da solução aquosa de fenol, ácido acético e glicerina sobre o tumor ascítico de Ehrlich. Estudo experimental in vitro. Acta Cirurgica Brasileira. 2004; 19(1):54-8. http://dx.doi.org/10.1590/S0102-86502004000100009.

Taghizadeh L, Brackman G, Nikiforov A, van der Mullen J, Leys C, Coenye T. Inactivation of biofilms using a low power atmospheric pressure argon plasma jet; the role of entrained nitrogen. Plasma Processes and Polymers. 2015; 12(1):75-81. http://dx.doi.org/10.1002/ppap.201400074.

Traba C, Liang JF. The inactivation of Staphylococcus aureus biofilms using low-power argon plasma in a layer-by-layer approach. Biofouling. 2015; 31(1):39-48. http://dx.doi.org/10.1080/08927014.2014.995643. PMid:25569189.

Vasconcelos LC, Sampaio FC, Albuquerque AJR, Vasconcelos LCS. Cell viability of Candida albicans against the antifungal activity of thymol. Brazilian Dental Journal. 2014; 25(4):277-81. http://dx.doi.org/10.1590/0103-6440201300052. PMid:25250489.
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