In this work interesting bioeffects have beem observed as consequence of sonication of cells of Sacharomyces Cerevislae. The irradiation of the cells at frequencies of 1,8mHz and intensities of 200 mW/cm2 callsed a slight activation of the enzymes acid phosphatase and ATPase from cellular envelope however irradiation at frequencies of 20 KHz and intensities of lOW/cm promoted a great activation of these enzymes. The experimental results confirm that the irradiation both at low and high frequencies submits the cell surface to high compression and rarefation forces that could disrupt the solvatation shell around cells and promote the influx of substance from medium to periplasmic space. When the same cells are sonicaled and transfered to distilated water it was observed a great delivering efflux of g1icose and ionic salts from cellular envelope suggeslion in this condition the yeast cells decrease the rate of development. It was observed lhat lhe mean volume of sonicaled cells was 35% smaller than not sonicated cells. The observed bioeffects suggesl lhat ultrasonic irradialion of cells in suspensions is a powerful tool to study its complex behaviour and may generate important biolechnological process in a near future.

In this work interesting bioeffects have beem observed as consequence of sonication of cells of Sacharomyces Cerevislae. The irradiation of the cells at frequencies of 1,8mHz and intensities of 200 mW/cm2 callsed a slight activation of the enzymes acid phosphatase and ATPase from ccllular envelopei however irradiation at frequencies of 20 KHz and intensities of lOW/cm promoted a great activation of these enzymes. The experimental results confirm that the irradiation both at low and high frequencies submits the cell surface to high compression and rarefation forces that could disrupt the solvatation shell around cells and promote the influx of substance from medium to periplasmic space. When the same cells are sonicaled and transfered to distilated water it was observed a great delivering efflux of g1icose and ionic salts from cellular envelope suggeslion in this condition the yeast cells decrease the rate of development. It was observed lhat lhe mean volume of sonicaled cells was 35% smaller than not sonicated cells. The observed bioeffects suggesl lhat ultrasonic irradialion of cells in suspensions is a powerful tool to study its complex behaviour and may generate important biolechnological process in a near future.